生物科学综合实验/张雁

Chapter 1 DNA Isolation Section 1 DNA in a Bottle Section 2 Isolation and Purification of Genomic DNA from Cheek Cells Section 3 DNA Isolation from Chicken Embryonic Allantoic Fluid Section 4 Know More Chapter 2 DNA Analysis Section 1 Do You Have Jumping Alu in Your Genome? Section 2 Boy or Girl? Section 3 Know More Chapter 3 RNA Extraction and RT-PCR Section 1 RNA Extraction and Purification Section 2 RT-PCR Section 3 TA Cloning Chapter 4 Bacterial Transformation and Plasrnid Purification Section 1 Bacterial Transformation and Culture Section 2 Colony PCR Screening for the Reconstructed Plasmids Section 3 Purification of Plasmid DNA Section 4 Know More Chapter 5 Protein Extraction Section 1 Preparation of Protein Section 2 Know More Chapter 6 Protein Analysis Section 1 SDS-PAGE and Western Blotting Section 2 Know More Chapter 7 Animal Cell Culture Section 1 Subculturing Cells Section 2 Cryopreservation Section 3 Recovery of Cryopreserved Cells Section 4 Know More Chapter 8 Cell Transfection Section 1 Transient Transfection Section 2 Detecting Transfection Efficiency by Flow Cytometry Chapter 9 Cell Contamination Section 1 Types of Cell Contamination Section 2 Detection of Contamination Section 3 Removal of Contamination Section 4 Know More Chapter 10 Genome Editing by CRISPR/Cas9 Section 1 CRISPR/Cas9 System Section 2 Cloning a gRNA into a Vector Section 3 Purification of Cas9/sgRNA Coexpression Vectors Section 4 Cotransfection of Cas9/sgRNA Coexpression Vectors into the Cells Section 5 PCR Analysis of Targeted Deletion in the Genome Section 6 Know More Chapter 11 Biosafety in Laboratory Section 1 General Laboratory Safety Section 2 Biosafety Chapter 12 The Laboratory Report Appendix Section 1 Experimental Instruments Section 2 Preparation of Commonly--Used Solutions