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- ISBN:9787030767691
- 装帧:圆脊精装
- 册数:暂无
- 重量:暂无
- 开本:其他
- 页数:1248
- 出版时间:2023-11-01
- 条形码:9787030767691 ; 978-7-03-076769-1
内容简介
本书为中国科学院院士李季伦科研论文著作选集。李季伦为中国农业大学生物学院教授。自1950年至今,一直工作在教学、科研**线。本书分别从四个主要的方面(生物固氮的研究、微生物次生代谢产物研发及生物合成调控研究、真菌毒素及生物能源的研究和趋磁螺菌及其纳米磁小体的研究),通过这四个方面的大约200余篇科学论文,记录了李季伦院士的科研思想和科研历程,以及对微生物学科所作出的学术贡献。由于论文数量较多,本书只选择性地收录了部分研究论文,充分体现了李季伦教授敏锐的科研洞察力和脚踏实地的科研特质,展示了他理论联系实际,为社会和大众服务的科研理念,善于发现和解决问题的工作作风;团结协作,勇于攻关的无私奉献精神。
目录
目录
序
前言
上
第1部分 生物固氮研究
我国生物固氮研究的现状和对策 4
花生根瘤菌自生固氮的研究 8
耐盐高效大豆根瘤菌株的构建 15
苜蓿根瘤菌 (Rhizobium meliloti) 的耐盐性研究 21
红豆草根瘤菌的耐盐和共生性状改造 28
Tn5-Mob系统诱导根瘤菌属之间耐盐和共生性状的转移 33
北京郊区固氮螺菌的研究 38
酶联免疫吸附技术(ELISA)对大豆根瘤菌的鉴定 46
肺炎克氏杆菌的nifA基因产物在巴西固氮螺菌中的功效 52
巴西固氮螺菌(Azospirillum brasilense)Yu62的质粒及nif HDK基因的定位 61
肺炎克氏杆菌nifA基因在巴西固氮螺菌固氮基因表达的铵调节中的作用 67
几种固氮菌nifA基因片段的同源性分析 71
巴西固氮螺菌ntrBC基因的克隆与核苷酸序列分析 76
肺炎克氏杆菌NifA对巴西固氮螺菌nifH启动子的转录激活作用 83
巴西固氮螺菌Yu62 draTG基因启动子区域的核苷酸序列及其功能分析 89
巴西固氮螺菌Yu62 draTG基因及其下游区域的定位诱变分析 97
巴西固氮螺菌Yu62 nifA基因克隆、测序及功能分析 103
巴西固氮螺菌鞭毛调节基因flbD鉴定 109
巴西固氮螺菌Yu62 glnB基因的克隆及其功能分析 115
玉米联合固氮工程菌的增产和节约氮肥的效果 122
Interaction Between PII and NifA in Azospirillum brasilense Sp7 126
Functional Analysis of the GAF Domain of NifA in Azospirillum brasilense: Effects of Tyr → Phe Mutations on NifA and Its Interaction with GlnB 133
PAS Domain of the Deduced Org35 Protein Mediates the Interaction with NifA 144
Isolation of a Flagellar Operon in Azospirillum brasilense and Functional Analysis of FlbD 149
Interaction Between GlnB and the N-terminal Domain of NifA in Azospirillum brasilense 161
A Minimal Nitrogen Fixation Gene Cluster from Paenibacillus sp. WLY78 Enables Expression of Active Nitrogenase in Escherichia coli 171
Comparative Genomic Analysis of N2-Fixing and Non-N2-Fixing Paenibacillus spp.: Organization, Evolution and Expression of the Nitrogen Fixation Genes 188
Positive and Negative Regulation of Transferred nif Genes Mediated by Indigenous GlnR in Gram-positive Paenibacillus polymyxa 212
Combined Assembly and Targeted Integration of Multigene for Nitrogenase Biosynthetic Pathway in Saccharomyces cerevisiae 234
Influence of pN2 and pD2 on HD Formation by Various Nitrogenases 248
固氮酶催化的放H2反应 265
肺炎克氏杆菌固氮酶双突变株的构建及其对底物还原的特性 275
N2 和H+在固氮酶活性中心金属原子簇中还原位点的分析 285
Mutagenesis at α-423Ile of MoFe Protein Reduces the Catalytic Activity of Nitrogenase in Klebsiella oxytoca 294
α-Lys424 Participates in Insertion of FeMo-co to MoFe Protein and Maintains Nitrogenase Activity in Klebsiella oxytoca M5al 305
第2部分微生物次生代谢产物研发及生物合成调控研究
北京农业大学的赤霉素研究工作(简报) 326
赤霉素A4、A7的发酵研究 330
赤霉素A9检定方法的建立和发酵的初步研究 337
莫能菌素应用技术 343
透明颤菌血红蛋白基因在阿维链霉菌中的表达 345
阿维菌素高产菌株的选育及阿维菌素B1的鉴定 352
Avermectins发酵培养基的研究 358
阿维链霉菌中aveD基因缺失对阿维菌素合成的影响 366
Deletion Analysis of Oligomycin PKS Genes (olmA) in Streptomyces avermitilis 371
Construction of Ivermectin Producer by Domain Swaps of Avermectin Polyketide Synthase in Streptomyces avermitilis 380
An adpA Homologue in Streptomyces avermitilis is Involved in Regulation of Morphogenesis and Melanogenesis 392
Engineering of Avermectin Biosynthetic Genes to Improve Production of Ivermectin in Streptomyces avermitilis 403
Enhancement of Avermectin and Ivermectin Production by Overexpression of the Maltose ATP-binding Cassette Transporter in Streptomyces avermitilis 410
A New Strain of Streptomyces avermitilis Produces High Yield of Oligomycin A with Potent Anti-tumor Activity on Human Cancer Cell Lines in vitro 423
Chromosomal Instability in Streptomyces avermitilis: Major Deletion in the Central Region and Stable Circularized Chromosome 433
The Pathway-specific Regulator AveR from Streptomyces avermitilis Positively Regulates Avermectin Production while it Negatively Affects Oligomycin Biosynthesis 450
Increasing Avermectin Production in Streptomyces avermitilis by Manipulating the Expression of a Novel TetR-family Regulator and Its Target Gene Product 466
AvaR2, a Pseudo γ-Butyrolactone Receptor Homologue from Streptomyces avermitilis, is a Pleiotropic Repressor of Avermectin and Avenolide Biosynthesis and Cell Growth 490
SAV742, a Novel AraC-Family Regulator from Streptomyces avermitilis, Controls Avermectin Biosynthesis, Cell Growth and Development 513
AvaR1, a Butenolide-type Autoregulator Receptor in Streptomyces avermitilis, Directly Represses Avenolide and Avermectin Biosynthesis and Multiple Physiological Responses 532
Redox-sensing Regulator Rex Regulates Aerobic Metabolism, Morphological Differentiation, and Avermectin Production in Streptomyces avermitilis 552
The PhoP Transcription Factor Negatively Regulates Avermectin Biosynthesis in Streptomyces avermitilis 568
IdeR, a DtxR-family Iron-response Regulator, Controls Iron Homeostasis, Morphological Differentiation, Secondary Metabolism, and Oxidative Stress Response in Streptomyces avermitilis 583
AveI, an AtrA Homolog of Streptomyces avermitilis, Controls Avermectin and Oligomycin Production, Melanogenesis, and Morphological Differentiation 604
SufR, a [4Fe-4S] Cluster-containing Transcription Factor, Represses the sufRBDCSU Operon in Streptomyces avermitilis Iron-sulfur Cluster Assembly 621
The ROK-family Regulator Rok7B7 Directly Controls Carbon Catabolite Repression, Antibiotic Biosynthesis, and Morphological Development in Streptomyces avermitilis 642
AccR, a TetR Family Tra
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