植物生理学实验实训综合教程(中英双语版)

中文篇 第1章 植物生理学实验基础 3 第2章 植物种子的萌发 14 第3章 植物水分代谢 22 第4章 矿质元素缺乏对植物生命活动的影响 30 第5章 植物光合性能的评价 42 第6章 植物碳氮代谢 59 第7章 植物生长物质的生理效应及其对植物生长发育的影响 72 第8章 植物组织培养 83 第9章 植物逆境生理 95 附　录 115 附录1 相关理论知识巩固与思考115 附录2 化学品标志符号202 附录3 常用酸碱的浓度203 附录4 常用固态酸、碱、盐的物质的量浓度配制参考 203 附录5 常用缓冲液的配制204 附录6 常用酸碱指示剂209 附录7 标准计量单位210 附录8 常用植物生长物质的一些化学特性212 参考文献 213 英文篇 Chapter 1 Experimental Basis of Plant Physiology 217 11 Plant physiology laboratory rules217 12 Classification and preparation methods of chemical reagent218 13 Collection, processing and preservation of experimental material220 14 Basic methods of experimental studies on plant physiology228 Chapter 2 Plant Seed Germination 232 21　 Determination of seed germination rate, germination potential and germination index233 22　 Rapid determination of seed viability234 23　 Determination of seed vigor236 24　 The formation and determination of the amylase activity during seed germination238 25　 Changes in fatty acid content of oil seeds during germination239 26　 Changes in amino acid content during seed germination241 Chapter 3　 Plant Water Metabolism 243 31　 Determination of plant leaf water content, relative water content and water saturation deficit244 32　 Determination of free and bound water content in plant leaves (Malincic method) 245 33　 Determination of plant tissue water potential (Small flow method) 247 34　 Determination of plant tissue osmotic potential (Plasma wall separation method) 249 35　 Determination of transpiration rate251 Chapter 4　 Effects of Mineral Element Deficiency on Plant Life Activities 254 41　 Element deficiency symptoms in plants (solution culture) 255 42　 Determination of nitrate reductase activity259 43　 Determination of root activity (α - naphthylamine oxidation method) 261 44　 Determination of nitrate nitrogen in plants263 45　 Determination of plant phosphorus (molybdenum blue method) 265 Chapter 5　 Evaluation of Plant Photosynthetic Performance 268 51　 Extraction and physicochemical properties of photosynthetic pigments269 52　 Separation of photosynthetic pigments and determination of absorption spectra271 53　 Determination of chloroplast pigment content273 54　 Determination of plant leaf photosynthetic rate and gas exchange parameters 276 55　 Determination of chlorophyll fluorescence parameters280 56　 Determination of ribulose diphosphate carboxylase (RuBPCase) activity286 57　 Determination of phosphoenolpyruvate carboxylase (PEPCase) activity289 Chapter 6　 Plant Carbon and Nitrogen Metabolism 291 61　 Determination of sucrose, glucose and fructose content292 62　 Determination of free amino acid content294 63　 Determination of sucrose synthase and sucrose phosphatase activities296 64　 Determination of α - amylase and β - amylase activities298 65　 Determination of glutamine synthetase activity302 66　 Determination of soluble protein content304 Chapter 7　 The Physiological Effects of Plant Growth Substances and Their Effects on Plant Development 307 71　 Effects of auxin on the growth of wheat root and shoot308 72　 Effects of gibberellin on the growth of wheat seedlings310 73　 Effect of ethephon on the fruit ripening310 74　 Abscissive effects of abscisic acid (ABA) on plant petioles311 75　 Effects of gibberellin (GA3) and abscisic acid (ABA) on seed germination313 76　 Synthesis of α - amylase in seed induced by gibberellin (GA3) 314 77　 Effects of abscisic acid (ABA) on stomatal movement317 78　 Determination of phytohormones by liquid chromatography318 Chapter 8　 Plant Tissue Culture 320 81　 Preparation of culture medium321 82　 Sterilization, disinfection and inoculation325 83　 Subculture and propagation330 84　 Acclimation, transplanting and management of tube seedlings334 Chapter 9　 Plant Stress Physiology 336 91　 Determination of proline content337 92　 Determination of total flavonoid content339 93　 Determination of ascorbic acid (AsA, AA) 341 94　 Determination of glutathione (GsH) 345 95　 Determination of cytoplasmic membrane permeability - conductance method347 96　 Identification of degree of membrance peroxidation - determination of malondialdehyde content (MDA)349 97　 Identification of cell death in plant tissues (Trypan blue staining method) 351 98　 Determination of hydrogen peroxide (H2O2) content352 99　 Determination of superoxide anion production rate354 910　 Determination of superoxide dismutase (SOD) activity356 911　 Determination of peroxidase (POD) activity358 912　 Determination of catalase (CAT) activity360