分子生物学实验指导:a students guide

experiment 1  preparation of plasmid dnaexperiment 2  restriction enzyme digestion and agarose gel electrophoresisexperiment 3  polymerase chain reaction(pcr)experiment 4  dna purification and construction of recombinant dnaexperiment 5  preparation of competent e.coli cellsexperiment 6  transformation of recombinant dnaexperiment 7  identification and characterization of positive clonesexperiment 8  isolation oftotal rna from animal tissuesexperiment 9  molecular hybridizationexperiment 10  reverse transcription—polymerase chain reaction(rt-pcr) and ta-cloningexperiment 11  expression and purification of a fusion protein from e.coliexperiment 12  electrophoretic mobility gel shift assay(emsa)to detect dna—protein interactionsappendix i  brief introduction to the ncbi databaseappendix ii  vector informationappendix iii  instructions on how to use the equipmentreferences