生物化学实验-(双语)

前言
第l部分  生物化学实验基本原理与方法
part l basic principles and methodologies of biochemicalexperiments
  i生物大分子的分离与纯化的一般步骤与方法
  i general procedures and methods for isolation andpurification of
  biomacromolecules
  11分光光度法
  ii spectrophotometry
  iii层析
  iii chromatography
  iv电泳
  ⅳelectr叩horesis
  v离心
  v centrifugation
  v1分子生物学技术基础——聚合酶链式反应(pcr)
  vi brief introduction to basic technique of molecularbiologypolymerase
  chain reaction(pcr、
第2部分  基础性实验
part 2 basic experiments
  实验1蛋白质和氨基酸的呈色反应
  experiment l  color reactions of proteins and aminoacids
  实验2蛋白质的沉淀与变性
  experiment 2 precipitation and denaturationofproteins
  实验3微量凯氏定氮法测蛋白质总氮含量
  experiment 3 quantification of crude proteins—kieldahl’snitrogen
  determination
  实验4双缩脲法测定蛋白质含量
  experiment 4 quantification ofproteins—biulet method
  实验5 folin一酚试剂法测定蛋白质含量
  experiment 5 quantification ofproteins———folin—lowrymethod
  实验6紫外吸收法测定蛋白质含量
  experiment 6 quantification ofproteins by ultravioletabsorption
  实验7染料结合法测定蛋白质含量
  experiment 7 quantification ofproteins by dye—bindingmethod
  实验8几种重要氨基酸(赖氨酸、色氨酸和脯氨酸)的含量测定
  experiment 8 determination of the contents of someimportant amino acids
  (lystrp and pro)
  实验9血清蛋白的醋酸纤维薄膜电泳
  experiment 9 cellulose acetate membrane electrophoresisof serum proteins
  实验10酪蛋白的制备
  experiment 10 preparation ofcasein
  实验11  sds聚丙烯酰胺凝胶电泳(page)~ij定蛋白质分子质量
  experiment 11 molecular weight determination ofproteinsby sdspage
  实验12聚丙烯酰胺凝胶等电聚焦测定蛋白质等电点
  experiment 12 pi determination of proteins bypolyacrylamide gel
  isoelectrofocusing
  实验13酶的特性
  experiment 13 properties ofenzymes
  实验14多酚氧化酶米氏常数(‰)的测定
  experiment 14 determination of michaelis constant(kin)ofpolyphenol
  oxidase
  实验1 5核酸酶的活力测定
  experiment 15 activity assay ofnucleases
  实验16谷丙转氨酶活力测定
  experiment 16 determination ofactivity ofglutamate—pyruvate transaminase
  实验l 7植物抗氧化酶类(超氧物歧化酶、过氧化物酶和过氧化氢酶)活
  性测定
  experiment 17 activity assay of common antioxidantenzymes(sod，pod
  and cat)in plant
  实验1 8乳酸脱氢酶(ldh)活性测定
  experiment 18 activity assay oflactatedehydrogenase(ldh)
  实验19维生素c的定量测定——2，6二氯酚靛酚法
  experiment 19 quantification ofvitamin c by2，6dichlorophenolindo
  phenol method
  实验20花菜中核酸的分离和鉴定
  experiment 20 extraction and identification ofnucleicacids from
  cauliflower
  实验21酵母核糖核酸的分离及组分鉴定
  experiment 21 extraction ofyeast rna and identificationof its components
  实验22定磷法测定核酸含量
  experiment 22 quantification of nucleic acids by methodof phosphate
  determination
  实验23定糖法测定rna含量
  experiment 23  quantification of rna by ribosedetermination method
  实验24二苯胺法测定dna含量
  experiment 24 quantification of dna by diphenylaminemethod
  实验25紫外吸收法测定核酸含量
  experiment 25 quantification ofnucleic acids byultraviolet absorption
  method
  实验26醋酸纤维薄膜电泳分离核糖核苷酸
  experiment 26 separation of ribonucleotides by celluloseacetate membrane
  electrophoresis
  实验27  还原糖和总糖的含量测定_dns法
  experiment 27 determination of reducing sugar and totalsugar by dns
  method
  实验28血糖的定量测定——葡萄糖氧化酶氨基安替比林(cod—pap)
  experiment 28 quantification of blood glucose by glucoseoxidase4
  aminoantipyrine(god—pap)
  实验29粗脂肪的提取和测定
  experiment 29 extraction and quantitative determinationofcrude fat
  实验30  油脂的化学性质
  experiment 30 chemical properties ofoil or fat
第3部分  综合性实验
part 3  comprehensive experiments
  实验3l蛋白质的酸水解及其氨基酸的纸层析鉴定
  experiment 3 1  acid hydrolysis of proteins andisolation and identification
  ofamino acids by paper chromatography
  实验32离子交换层析分离混合氨基酸
  experiment 32  separating mixed amino acids byion—exchange
  chromatography
  实验33血清球蛋白的盐析分离及分子筛凝胶层析法脱盐
  experiment 33  saltingout separation of serumglobulins and desalting by
  molecular sieving gel chromatography
  实验34糖的测定和鉴定——蒽酮试剂和纸层析法
  experiment 34 quantification and identification ofsaccharides by anthrone
  colorimetry and paper chromatography
  实验35聚丙烯酰胺凝胶电泳分离乳酸脱氢酶同工酶
  experiment 35  separating ldh isoenzymes by page
  实验36叶绿体的制备及相关分析
  experiment 36 preparation and anabrses ofchloroplasts
  实验37植物分子生物学系列实验
  experiment 37 series experiments ofplant molecularbiology
参考文献
附录
  附录1  基础生物化学实验室常用仪器简介
  附录2  常用缓冲溶液的配制